文章基本信息

标题：Effect of Nitrogen and Carbon Sources on Lipase Production by Penicillium aurantiogriseum
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作者：Lima, Valéria M. G. ; Krieger, Nadia ; Sarquis, Maria Inez M. 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2003
卷号：41
期号：2
页码：105-110
语种：English
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：A wild fungal strain isolated from soybean oil and identified as Penicillium aurantiogriseum initially presented a volumetric lipase activity of 0.4 U/mL in submerged culture in a medium containing 0.5 % yeast extract and 1 % olive oil. Studies were undertaken to improve lipase production. The effect of nitrogen source was studied by adding casein peptone, meat peptone, yeast extract or ammonium sulfate to a medium containing potassium nitrate and other mineral salts. The best yield, of 13 U/mL after 72 h, was obtained with the medium supplemented with ammonium sulfate. With the ammonium sulfate concentration increased to double the C/N ratio from 2.5 to 5, a lipolytic activity of 18 U/mL was obtained. Olive, corn, soy and sunflower oils were tested as carbon sources in this medium, with olive oil at 1 % giving a lipolytic activity of 25 U/mL after 48 h, the highest yield obtained in this study. Enzyme production was best at 29 °C, within a range tested from 26 to 32 °C. These results are promising because this strain produces lipase in an inexpensive inorganic medium and we succeeded in increasing the lipolytic activity 62-fold over the initial values obtained with the non-optimized medium.
关键词：lipases; Penicillium aurantiogriseum; media optimization; lipolytic activity; inorganic nitrogen sources