文章基本信息

标题：Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
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作者：Sean W. Corley ; Nicholas N. Jonsson ; Emily K. Piper 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2013
卷号：110
期号：42
页码：16772-16777
DOI：10.1073/pnas.1309072110
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the {beta}-adrenergic octopamine receptor gene (Rm{beta}AOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the Rm{beta}AOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the Rm{beta}AOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the Rm{beta}AOR gene are likely to confer resistance to amitraz.