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标题：Thermomyces lanuginosus CBS 395.62/b Strain as Rich Source of α-Galactosidase Enzyme
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作者：Rezessy-Szabó, Judit M. ; Nguyen, Quang D. ; Bujna, Erika 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2003
卷号：41
期号：1
页码：55-59
语种：English
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Seventeen Thermomyces lanuginosus strains, cultivated on raffinose and sucrose, were ranked on the basis of α-galactosidase activities. T. lanuginosus CBS 395.62/b strain showed the highest α-galactosidase activity on both investigated carbohydrates. Several carbon sources were tested as potential inducers for the α-galactosidase synthesis. On melibiose substrate α-galactosidase activity was higher in the intracellular fraction than in the filtrate of the fermentation broth, although both values were very low and did not reach the value of 1 U/mL. Raffinose, sucrose and Lactosucrose® proved to be inducers for α-galactosidase production. The highest titer (about 30 U/mL) was achieved on 1 % sucrose and 0.45 % ammonium acetate. The optimum sucrose and ammonium acetate concentrations, at which about 90 U/mL α-galactosidase activity was reached during an 8-day fermentation, were 3 and 0.9 %, respectively.
关键词：α -galactosidase; fermentation; enzyme production; Thermomyces lanuginosus