文章基本信息

标题：Transformace hybridní osiky pomocí Agrobacterium tumefaciens.
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作者：P. Máchová ; H. Cvrčková ; J. Malá 等
期刊名称：Zprávy Lesnického Výzkumu
印刷版ISSN：0322-9688
出版年度：2007
卷号：52
期号：4
页码：354-360
出版社：Výzkumný Ústav Lesního Hospodárství a Myslivosti
摘要：The European aspen (Populus tremula) represents a very modest tree species in climatic conditions and soil quality. For its regeneration ability, it is a very perspective woody species for tissue culture manipulation, genetic engineering, and genetic mapping. Stem segments and leaves discs of the hybrid aspen (Populus tremula x P. tremuloides) clone no. 5 preserved in the FGMRI gene bank were used for testing of genetic transformation by agrobacterium tumefaciens. Transformed shoots were obtained using four agrobacterial constructs bearing uidA (GUS) gene governed by various promoters. Presence of nptII and GUs genes in transgenic tissues and plants was verified using the polymerase chain reaction.