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标题：The Use of Antibody-sensitized Latex to Detect Cymbidium Mosaic Virus in Orchids
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作者：Norani Abdul-Samad ; Zainab Ari
期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：1993
卷号：16
期号：2
出版社：Universiti Putra Malaysia
摘要：Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 µg/ml The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.