文章基本信息

标题：A Competitive ELISA for Quantification of Protein A in Culture Medium
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作者：Abdul Manaf Ali1 Sharifah Tahir ; Baharuddin Abdul Ghani ; Ungku Chulan 等
期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：1996
卷号：19
期号：2&3
出版社：Universiti Putra Malaysia
摘要：A competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG molecules between alkaline phosphatase-labelled protein A and unlabelled protein A. The optimum IgG concentration required for coating was 2-4 fig I ml. The optimum incubation time for colour development using (p-nitrophenol phosphate substrate was 20-30 min. The lowest protein A concentration that could be measured using the optimized competitive ELISA was 20 ng/ml, and the maximum 2 fig I ml. The amount of protein A produced in brain-heart infusion medium increased exponentially during log phase of cell growth, reaching a maximum concentration of 22.5 fig I ml after 15 h cultivation.
关键词：protein A; competitive ELISA; IgG; Staphylococcus aureus