文章基本信息

标题：A Protocol for Efficient Plantlet Regeneration from Leaf Derived Callus of Lablab Bean (Lablab purpureus var. lignosus (L) prain)
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作者：Muthu Thiruvengadam ; Narayanasamypillai Jayabalan
期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：2003
卷号：26
期号：1
出版社：Universiti Putra Malaysia
摘要：An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (1AA, 2, 4-D and NAA) and cytokinin (BAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and BAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0,5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of BAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.
关键词：Lablab purpureus; multiple shoot induction; organogenesis; tissue culture