文章基本信息

标题：Intraspecific Polymorphism in Mystus nemurus (C&V) Detected by RAPD-PCR Fingerprinting
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作者：Sanga Leesanga ; Siti Shapor Siraj ; Siti Khalijah Baud 等
期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：2004
卷号：27
期号：1
出版社：Universiti Putra Malaysia
摘要：Yellow catfish, Mystus nemurus (C&V), is becoming one of the major freshwater species farmed by aquaculturists in Southeast Asia. It was of interest to examine levels of genetic sub-population differentiation among samples of this species obtained from parts of its range, as well as to compare the genetics of mild and hatchery-bred fish. The genetic aspects of variation in the fish, which were collected from eight wild populations throughout Thailand and a hatchery stock, were determined at molecular (DNA) level using the technique of RAPD-PCR fingerprinting. Five arbitrary primers namely OPA-11, OPA-14, OPA-18, OPA-19 and OPA-20 were chosen to amplify products, which showed 28 polymorphic loci in 9 populations. The highest genetic distance (D) was found between Chainat and Suratthani populations with the value of 0.289, while the lowest was found in Songkhla population and hatchery stock with the value of 0.087. The dendrogram depicts the genetic relationship among populations of M. nemurus, which are grouped into four clusters according to their regional areas.
关键词：Mystus nemurus; catfish; RAPD; PCR; Thailand