文章基本信息

标题：Rapid and Non-radioactive Detection Method of Microsatellites in Mystus nemurus: A Refined Technique
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作者：Chan Soon Choy ; Siti Shapor Siraj ; Tan Soon Guan 等
期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：2005
卷号：28
期号：1
出版社：Universiti Putra Malaysia
摘要：A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellite marker development for the River catfish, Mystus nemurus. The current refined protocol for microsatellite isolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences being submitted to GenBank. This refined technique is able to reduce the total time required from PCR cloning till sequencing specific microsatellite regions to less than three and a half months
关键词：Microsatellites; Mystus nemurus; cloning; polymerase chain reaction (PCR); random amplified microsatellites (RAMs)