文章基本信息

标题：α-Amylase Production by Bacillus cereus MTCC 1305 Using Solid-State Fermentation
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作者：Anto, Hema ; Trivedi, Ujjval ; Patel, Kamlesh 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2006
卷号：44
期号：2
页码：241-245
语种：English
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Production of α-amylase under solid-state fermentation by Bacillus cereus MTCC 1305 has been investigated using wheat bran and rice flake manufacturing waste as substrates. With wheat bran, highest enzyme production expressed as units per mass of dry substrate ((94±2) U/g) was observed. Production parameters were optimized as inoculum size 10 % (volume per mass) and substrate:moisture ratio 1:1. Among different carbon sources supplemented, glucose (0.04 g/g) showed enhanced enzyme production ((122±5) U/g). Supplementation of different nitrogen sources (0.02 g/g) showed decline in enzyme production. Optimum α-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C, enzyme showed 90 % activity compared to 55 °C.
关键词：α-amylase; Bacillus cereus; solid-state fermentation; wheat bran; optimization