文章基本信息

标题：TILLING: A new biotechnological method for protection and improvement of durum wheat
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作者：El Yadini M. ; Kahama I. ; Labhilili M. 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2013
卷号：4
期号：6
页码：931-934
出版社：University of Mohammed Premier Oujda
摘要：A new reverse genetic method (Targeting Induced Local Lesion In Genom ) was developed on 1357 individuals durum wheat variety Cham 1 mutated. The seeds of studied population were treated with 0,6% (v/v) EMS, and advanced to the stage of generation M8. Screening of mutations in the HMW-GS and LMW-GS was performed using one-dimensional SDS-PAGE and molecular marker. The results showed eight mutants detected in LMW-GS. Those mutants showed a different profile compared to that of the parent Cham1 unmutated. PCR with a specific primer was used as a tool of confirmation of those results, by amplification of LMW-GS region and differentiated between the profile of good or bad technological properties of durum wheat
关键词：Mutations ; glutenins ;EMS; TILLING; LMW;GS