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标题：Genetic Variation among European Lophodermium piceae Populations - Preliminary Results
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作者：Michael M. MüLLER ; Thomas N. SIEBER ; Jarkko HANTULA 等
期刊名称：Acta Silvatica et Lignaria Hungarica
印刷版ISSN：1786-691X
电子版ISSN：1787-064X
出版年度：2007
卷号：SPECIAL
页码：61-64
出版社：Forestry Commission of the Hungarian Academy of Sciences
摘要：Lophodermium piceae is a common needle endophyte of Norway spruce (Picea abies). The aim of the present study was to examine the degree of differentiation within and among European populations separated by various distances and geographical obstacles. For this purpose, populations (including > 10 isolates/subpopulation) were collected along a north-south transect stretching from the northern timberline in Finnish Lapland to the southern border of the distribution area of Norway spruce in northern Italy. Differentiation between L. piceae populations was determined from DNA sequences of three genetic markers. One of the markers was the internal transcribed spacer (ITS) of the ribosomal DNA and the other two (LP1 and LP2) were based on sequence characterized amplified regions (SCAR) designed for L. piceae. Preliminary results including sequences of Finnish, Swiss and Italian isolates show low differentiation among populations. According to analysis of molecular variance the among population variation was 1%, 5% and 0% in ITS, LP1 and LP2 markers, respectively.
关键词：Norway spruce ; Picea abies ; endophyte ; genetic differentiation ; gene flow