文章基本信息

标题：Placental Mitochondrial DNA Content and Particulate Air Pollution during in Utero Life
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作者：Bram G. Janssen ; Elke Munters ; Nicky Pieters 等
期刊名称：Environmental Health Perspectives
印刷版ISSN：0091-6765
电子版ISSN：1552-9924
出版年度：2012
卷号：120
期号：9
页码：1346-1352
DOI：10.1289/ehp.1104458
出版社：OCR Subscription Services Inc
摘要：a c k g r o u n d: Studies emphasize the importance of particulate matter (PM) in the formation of reactive oxygen species and inflammation. We hypothesized that these processes can influence mito-chondrial function of the placenta and fetus.oB j e c t i v e: We investigated the influence of PM10exposure during pregnancy on the mitochon-drial DNA content (mtDNA content) of the placenta and umbilical cord blood.Me t h o d s: DNA was extracted from placental tissue (n = 174) and umbilical cord leukocytes (n = 176). Relative mtDNA copy numbers (i.e., mtDNA content) were determined by real-time polymerase chain reaction. Multiple regression models were used to link mtDNA content and in utero exposure to PM10over various time windows during pregnancy.re s u l t s: In multivariate-adjusted analysis, a 10-μg/m3 increase in PM10exposure during the last month of pregnancy was associated with a 16.1% decrease [95% confidence interval (CI): –25.2, –6.0%, p = 0.003] in placental mtDNA content. The corresponding effect size for average PM10exposure during the third trimester was 17.4% (95% CI: –31.8, –0.1%, p = 0.05). Furthermore, we found that each doubling in residential distance to major roads was associated with an increase in placental mtDNA content of 4.0% (95% CI: 0.4, 7.8%, p = 0.03). No association was found between cord blood mtDNA content and PM10exposure.co n c l u s i o n s: Prenatal PM10exposure was associated with placental mitochondrial alterations, which may both reflect and intensify oxidative stress production. The potential health consequences of decreased placental mtDNA content in early life must be further elucidated
关键词：fetal development; mitochondrial DNA content; mitochondrial function; particulate ;matter.