文章基本信息

标题：Toxic Effects of Nano-CuO, Micro-CuO and Cu²⁺ on Chlorella sp.
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作者：Liyan Wang ; Mian Wang ; Changsheng Peng 等
期刊名称：Journal of Environmental Protection
印刷版ISSN：2152-2197
电子版ISSN：2152-2219
出版年度：2013
卷号：4
期号：1B
页码：86-91
DOI：10.4236/jep.2013.41B016
出版社：Scientific Research Publishing
摘要：The 96 h acute toxic effects of nano-CuO (N-CuO), micro-CuO (M-CuO) and ²⁺ on Chlorella sp. were investigated in this paper. The results showed that toxicities decreased in an order of Cu²⁺＞N-CuO＞M-CuO. The 96 h EC₅₀ of Cu²⁺ on Chlorella sp. was 1.06 mg /L, and of N-CuO it was 74.61 mg /L, while no pronounced toxicity was observed when the concentration of M-CuO was lower than 160 mg/L. Further experiments were carried out in order to study the toxicity mechanism of nano-CuO on Chlorella sp.. The results of Cu²⁺ release from N-CuO showed less than 0.2 mg/L Cu2+ were released, so the release of Cu²⁺ was not responsible for the toxicity. Further experiments showed N-CuO inhibited formation of Chlorophyll A. Content of Chlorophyll A in the control group was 4.75 mg/10⁸ cells, while it declined to 2.89 mg/10⁸ cells for 160 mg/L N-CuO after 96 h, which indicated that N-CuO could inhibit photosynthesis of Chlorella sp.. Moreover, N-CuO condensed with algal cells. It affected the activity of SOD and POD, indicating that N-CuO could cause oxidant stress to Chlorella sp.. These may be the toxicity mechanism.
关键词：Nano-CuO; Chlorella sp.; Toxic effects; Photosynthesis; Oxidant stress