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标题：A Modified Selective Medium Containing Benzalkonium Chloride (BKC) for the Isolation of Pseudomonas aeruginosafrom Raw Milk
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作者：Aylin Akoglu ; Evrim Gunes Altuntas ; Gokce Polat Yemis 等
期刊名称：Food and Nutrition Sciences
印刷版ISSN：2157-944X
电子版ISSN：2157-9458
出版年度：2012
卷号：3
期号：7
页码：947-950
DOI：10.4236/fns.2012.37125
出版社：Scientific Research Publishing
摘要：A modified selective medium (modified Cetrimide Agar, mCA) consisting of 200 µg/mL benzalkonium chloride (BKC) was developed for the isolation of Pseudomonas aeruginosa from raw milk. Initially, a total of 55 isolates were obtained from 14 raw milk samples collected from several dairy plants in Ankara, Turkey. Among these isolates, 19 were identified as Pseudomonas aeruginosa, 28 as Pseudomonas fluorescens, 4 as Acinetobacter baumannii, 2 as Enterobacter intermedium, 1 asEnterobacter agglomerans, and 1 as Escherichia coli using Microbact biochemical test kit. BKC was chosen as a selective agent to suppress growth of competitive flora because it is very effective against a wide range of Gram-negative bacteria while P. aeruginosa is resistant. MICs (minimum inhibitory concentration) for BKC were determined by agar dilution method. The concentration of 200 µg/mL BKC inhibited competitive flora, while 90% of P. aeruginosa strains were resistant. When the results of enumeration of P. aeruginosa and other Gram (-) bacteria in Cetrimide Agar (CA) and mCA were compared, it was observed that mCA was more selective than the standard CA in preventing the growth of competitive flora especially of P. fluorescens.
关键词：P. aeruginosa; Benzalkonium Chloride (BKC); Modified Cetrimide Agar; Raw Milk