文章基本信息

标题：COMPARATIVE DNA DEGRADATION IN BONES FRAGMENTS
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作者：Lucian GORGAN ; Radu DRUICĂ ; Andrei ŞTEFAN 等
期刊名称：International Journal of Criminal Investigation
电子版ISSN：2247-0271
出版年度：2013
卷号：3
期号：1
页码：33-43
出版社：AIT Laboratories
摘要：The aim of this study is to follow the changes that occur, in time, at DNA level and to establish an efficient and reliable protocol for ancestral DNA extraction from bones found in archaeological sites. To test whether the protocol is efficient and capable of yielding good quality DNA, extraction was first performed on fresh bones. The material consists of fresh pig (Sus scrofa) and cow (Bos taurus) bones that were grounded by using a drill operating at low speed and the bone powder was incubated in lysis buffer in the presence of proteinase K. DNA isolation and purification were done by using the phenol: chloroform protocol and DNA was precipitated with absolute ethanol stored at -20oC. The extractions were carried out once every month for a total of four extractions
关键词：DNA; bone tissue; ancient DNA; total DNA