文章基本信息

标题：Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers
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作者：Paulina D. Rakowska ; Haibo Jiang ; Santanu Ray 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2013
卷号：110
期号：22
页码：8918-8923
DOI：10.1073/pnas.1222824110
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although the direct observation of the fundamental processes is lacking. By combining rational peptide design with topographical (atomic force microscopy) and chemical (nanoscale secondary ion mass spectrometry) imaging on the same samples, we show that pores formed by antimicrobial peptides in supported lipid bilayers are not necessarily limited to a particular diameter, nor they are transient, but can expand laterally at the nano-to-micrometer scale to the point of complete membrane disintegration. The results offer a mechanistic basis for membrane poration as a generic physicochemical process of cooperative and continuous peptide recruitment in the available phospholipid matrix.
关键词：innate host defense ; de novo protein design ; nanometrology ; antibiotics ; nanoscopy