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标题：Comparative reproducibility of SYBR Green I and TaqMan real-time PCR chemistries for the analysis of matrix and hemagglutinin genes of Influenza A viruses
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作者：Binod Kumar ; Prashant Kumar ; Roopali Rajput 等
期刊名称：International Journal of Collaborative Research on Internal Medicine & Public Health
电子版ISSN：1840-4529
出版年度：2012
卷号：4
期号：7
出版社：DRUNPP Sarajevo
摘要：Background: The outbreak of novel influenza A H1N1-2009 virus (pH1N1) and its rapid spread worldwide raised serious concern about pandemic preparedness. Objectives: The present study was designed to evaluate the sensitivity and specificity of two chemistries of real-time RT-PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for detection of the matrix and hemagglutinin genes of human influenza A viruses. Methods: Influenza A virus reference strains were used to perform the calibration curve analysis and the diagnostic accuracy of both the real-time RT-PCR formats were assessed on 110 clinical specimens from patients presenting with influenza-like-illness (ILI).Result: The study results suggest that the TaqMan chemistry has better sensitivity as compared to SYBR Green I, however, the SYBR Green I assay was found to be simpler, economical and readily available. Conclusions: It is concluded that the SYBR Green I assay has equal potential to be considered as an alternative to the TaqMan assay as a preparedness measure for any future influenza outbreak management.
关键词：Influenza virus; Real-time PCR; TaqMan assay; SYBR Green assay