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标题：An alternative role of F_oF₁-ATP synthase in Escherichia coli: synthesis of thiamine triphosphate
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作者：Tiziana Gigliobianco ; Marjorie Gangolf ; Bernard Lakaye 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
DOI：10.1038/srep01071
出版社：Springer Nature
摘要：In E. coli , thiamine triphosphate (ThTP), a putative signaling molecule, transiently accumulates in response to amino acid starvation. This accumulation requires the presence of an energy substrate yielding pyruvate. Here we show that in intact bacteria ThTP is synthesized from free thiamine diphosphate (ThDP) and P_i, the reaction being energized by the proton-motive force (Δp) generated by the respiratory chain. ThTP production is suppressed in strains carrying mutations in F₁ or a deletion of the atp operon. Transformation with a plasmid encoding the whole atp operon fully restored ThTP production, highlighting the requirement for F_oF₁-ATP synthase in ThTP synthesis. Our results show that, under specific conditions of nutritional downshift, F_oF₁-ATP synthase catalyzes the synthesis of ThTP, rather than ATP, through a highly regulated process requiring pyruvate oxidation. Moreover, this chemiosmotic mechanism for ThTP production is conserved from E. coli to mammalian brain mitochondria.