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标题：Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
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作者：1Tatiana P. Yudina ; Elena V. Sorokina ; Mikhail M. Mazhul 等
期刊名称：European Researcher
印刷版ISSN：2219-8229
电子版ISSN：2224-0136
出版年度：2013
卷号：42
期号：2-3
出版社：European Researcher
摘要：The effects of silver ions on the luminescent recombinant strain of Escherichia coli carrying luxCDABE operon of Vibrio fischeri were investigated. The toxicity of silver ions was determined in 30 minutes and in chronic 24 hours experiments. Changes in the luminescence intensity and in the growth rate of bacteria were considered as a measure of silver ions toxicity within the range of concentrations applied. The effect of silver ions was demonstrated to be strongly dependent on the concentration of bacteria and on the medium composition. EC50 values were 0.018 mg/l after 30 min exposure and 0.014 mg/l after 10 hours of bacterial growth. Comparison of two modifications of the experiment showed that silver ions have a strong non-specific toxicity, as well as a specific effect on bacterial cells.
关键词：silver ions; bacterial luminescence test; E. Coli; toxicity; bacterial growth