文章基本信息

标题：Enhanced production of cellulase from tapioca stem using response surface methodology
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作者：Riswan Ali ; S.B. ; Muthuvelayuhdam R. 等
期刊名称：Innovative Romanian Food Biotechnology
电子版ISSN：1843-6099
出版年度：2013
期号：12
页码：40-51
出版社：Galati University Press
摘要：Medium composition for production of cellulase by Cellulomonas fimi NCIM-5015 from tapioca stem as a substrate in submerged fermentation was optimized using a stepwise strategy. Plackett-Burman design was employed for screening, the most significant nutrient components which influence the cellulose production from 12 nutrient components. Results revealed that peptone, yeast extract, KH2PO4and CaCl2.2H2O, have significant effects on cellulase production, with confidence levels above 95%. Central composite design (CCD) was used to determine the optimal concentrations of these four components and the experimental results were fitted with a second-order polynomial model at 95% level (P < 0.05). The optimum conditions are peptone (0.957 g/L), yeast extract (2.5 g/L), KH2PO4(3.09 g/L), and CaCl2 .2H2O (0.409 g/L). Under these conditions, the production of cellulase was found to be 0.58 IU/ml.
关键词：cellulase; Plackett-Burman Design; tapioca stem; response surface methodology