The paper is devoted to consideration of self-regulation training that can be used in order to prevent a development of negative human functional states (HFS) in work environment. According to the well-developed in Russian work and organizational psychology traditions the effects of practical implementation of various self-regulation methods on job efficiency and worker's well-being are analyzed. The positive patterns of changes in HFS syndromes taken place as a result of prolonged courses of self-regulation training are described. It is shown that a successful acquisition of self-regulation skills strongly depends on (1) specificity of job content and (2) individual sensitivity to various relaxation techniques. These findings are useful for enhancing the quality of work-site stress management and health promotion programs.