文章基本信息

标题：Quantification of Lung Damage in an Elastase-Induced Mouse Model of Emphysema
本地全文：下载
作者：Arrate Muñoz-Barrutia ; Mario Ceresa ; Xabier Artaechevarria 等
期刊名称：International Journal of Biomedical Imaging
印刷版ISSN：1687-4188
电子版ISSN：1687-4196
出版年度：2012
卷号：2012
DOI：10.1155/2012/734734
出版社：Hindawi Publishing Corporation
摘要：Objective. To define the sensitivity of microcomputed tomography- (micro-CT-) derived descriptors for the quantification of lung damage caused by elastase instillation. Materials and Methods. The lungs of 30 elastase treated and 30 control A/J mice were analyzed 1, 6, 12, and 24 hours and 7 and 17 days after elastase instillation using (i) breath-hold-gated micro-CT, (ii) pulmonary function tests (PFTs), (iii) RT-PCR for RNA cytokine expression, and (iv) histomorphometry. For the latter, an automatic, parallel software toolset was implemented that computes the airspace enlargement descriptors: mean linear intercept and weighted means of airspace diameters (, , and ). A Support Vector Classifier was trained and tested based on three nonhistological descriptors using as ground truth. Results. detected statistically significant differences between the groups at all time points. Furthermore, at 1 hour (24 hours) was significantly lower than at 24 hours (7 days). The classifier trained on the micro-CT-derived descriptors achieves an area under the curve (AUC) of 0.95 well above the others (PFTS AUC = 0.71; cytokine AUC = 0.88). Conclusion. Micro-CT-derived descriptors are more sensitive than the other methods compared, to detect in vivo early signs of the disease.