Rural ecomuseum is a natural and dynamic site, representing the unique and precious identity of a rural area. Ecomuseums introduce, manage, and preserve the cultural and natural heritage of the local communities. Therefore, in this paper zoning is carried out by multi-criteria evaluation method for developing rural ecomuseums in Mazandaran province rural areas. First of all, based on both local and foreign experiences, influential criteria in the zoning process were identified. In the next step, the criteria were prioritized with the help of Delphi method and AHP method. Then, given the criteria of paramount importance and the indicators of their constraining sub-criteria, potential countryside area for developing rural ecomuseums were determined by eliminating the regions with constraining criteria. Last of all, after analyzing the potential countryside areas, they were prioritized for creating rural ecomuseums. In the present study, 6 criterion groups, 20 main criteria, and 77 sub-criteria were used in the zoning process, the most important criteria being tourist, environmental, anthropological, aesthetic, architectural, and economic criteria. The findings show that the sites of priority for creating ecomuseums are Barase, Garsmasar, Lavij, Ab-e-Ask, Lajim, Kojoor, Kandelous, Ab-e-Garm, Veresk, Javaher-Deh, Asiab-Sar, Yoosh, Oskoo-Mahalle and Imamzadeh Abdullah, Hendoo-Kola, Farah-Abad, Kord-Kola, and Gol-Mahalleh.