文章基本信息

标题：Antibody Functionalised Gold Nanoprobes Based Colorimetric Assay for the Direct Detection of Phenylurea Herbicide
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作者：Priyanka Sharma ; Bipul Kumar ; C. Raman Suri 等
期刊名称：Environment and Pollution
印刷版ISSN：1927-0909
电子版ISSN：1927-0917
出版年度：2012
卷号：1
期号：1
页码：12
DOI：10.5539/ep.v1n1p12
语种：English
出版社：Canadian Center of Science and Education
摘要：One-step homogeneous colorimetric immunoassay format for direct detection of herbicide diuron is reported. Gold nanoparticles (30 nm) functionalised with specific anti-diuron antibody was used as bioprobes for the development of non-crosslinking hybridization method, where aggregation of the gold-nanoprobes is induced by an increasing salt concentration, and is prevented by the amount of antigen (diuron) present in sample solution. The aggregation profile of the antibody functionalised gold nanoparticles directed by the immunoreaction was investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS) and was further confirmed colorimetrically by measuring the change in the absorption ratio (620/520 nm) with increasing amounts of diuron. The assay exhibited an excellent sensitivity and specificity based on absorbance profile showing the dynamic response range from 0.1-50 ng/mL for diuron with a detection limit of ~5 ng/mL. The new technique could be used for fast, high-throughput screening of pesticides in environmental diagnostics at a very low cost.