The aim of this study is to assess the self-perception of pre-service teachers on leadership after getting involved into a Cooperative Learning approach. For that purpose, a pre/post-test has been applied to 57 undergraduate students enrolled in a unit course on Natural and Social Science Education, compulsory in the curriculum of a degree in Primary Education. The inventory of the questionnaire included scales referring to the skills of teamwork, self-understanding, communication, decision making and leadership, regarded as essential for their future professional development. Results show that students perceive higher levels in all of them after a Cooperative Learning experience, which suggests the positive impact of cooperative student-centered teaching strategies to develop such important skills.