文章基本信息

标题：OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD
本地全文：下载
作者：Pavol Bajzík ; Radoslav Židek ; Jozef Golian 等
期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2011
卷号：5
期号：3
DOI：10.5219/154
出版社：Association HACCP Consulting
摘要：European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.