文章基本信息

标题：Ethanol re-precipitation removes PCR inhibitors from Ancient DNA extract
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作者：PANDEY RAJEEV KUMAR ; SINGH PRATAP DEEPANKAR ; SUDHAKAR GODI 等
期刊名称：Antrocom : Online Journal of Anthropology
电子版ISSN：1973-2880
出版年度：2011
卷号：7
期号：2
出版社：Antrocom Onlus
摘要：One of the major problems in ancient DNA work is the presence of inhibitory substances, which hampers Taq polymerase activity. Therefore analysis of ancient DNA sample is very challenging. Here we describe a simple and competent ethanol re-precipitation based protocol for the purification of DNA from ancient bones and tissues. The efficiency of this procedure has been demonstrated on 600 years old biological samples provided by Anthropological Survey of India (Himalaya region). This suggests that re-precipitation of ancient DNA extracts removes PCR inhibitors and increases the success rate of amplification.