文章基本信息

标题：Purification and Characterization of Antioxidant Peptide from Sunflower Protein Hydrolysate
本地全文：下载
作者：Ren, Jian ; Zheng, Xi-Qun ; Liu, Xiao-Lan 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2010
卷号：48
期号：4
页码：519-523
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Sunflower proteins were hydrolyzed with Flavourzyme for the production of antioxidant peptide. DEAE-Sepharose Fast Flow, Sephadex G-25 gel filtration chromatography and reversed-phase HPLC were consecutively employed to purify a novel sunflower antioxidant peptide, and the ability to inhibit the autoxidation of pyrogallol was expressed as the antioxidative activity of the peptide. The amino acid sequence was identified as Ala-Cys-Ala-His-Asp-Lys-Val by a Q-Tof2 mass spectrometer. This novel peptide exhibited a high antioxidative activity of 79.42 U/mL, which is expected to protect against oxidative damage in living systems in relation to aging and carcinogenesis. Higher antioxidative activities were presumed mainly due to the presence of hydrophobic amino acids in its sequence.
关键词：antioxidant peptide; sunflower; protein hydrolysate; purification and characterization