首页    期刊浏览 2024年11月30日 星期六
登录注册

文章基本信息

  • 标题:Improvement of Folate Biosynthesis by Lactic Acid Bacteria Using Response Surface Methodology
  • 本地全文:下载
  • 作者:Nor, Norfarina Muhamad ; Mohamad, Rosfarizan ; Foo, Hooi Ling
  • 期刊名称:Food Technology and Biotechnology
  • 印刷版ISSN:1330-9862
  • 电子版ISSN:1334-2606
  • 出版年度:2010
  • 卷号:48
  • 期号:2
  • 页码:243-250
  • 出版社:Sveuciliste u Zagrebu - Faculty of Food
  • 摘要:Lactic acid bacteria (Lactococcus lactis NZ9000, Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 µg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 µM.
  • 关键词:folate; lactic acid bacteria; Lactobacillus plantarum I-UL4; response surface methodology
国家哲学社会科学文献中心版权所有