文章基本信息

标题：Characterization and Antioxidant Properties of Hemp Protein Hydrolysates Obtained with Neutrase®
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作者：Wang, Xian-Sheng ; Tang, Chuan-He ; Chen, Ling 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2009
卷号：47
期号：4
页码：428-434
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Hemp protein hydrolysates with various yields of trichloroacetic acid (TCA)-soluble peptides (Ysp) and surface hydrophobicity (Ho) were obtained by Neutrase® hydrolysis from hemp protein isolate (HPI). The peptide profiles, amino acid composition and antioxidant activities (DPPH radical scavenging ability, reducing power and Fe2+ chelating ability) of the hydrolysates, obtained at 60–240 min, were evaluated. Higher DPPH radical scavenging (IC50, 2.3–2.4 mg/mL) and Fe2+ chelating (IC50, 1.7–1.8 mg/mL) abilities were observed for the hydrolysates with Ysp in the range of 28–30 and 18–28 %, respectively, while the high reducing power was only observed for the hydrolysate with Ysp of 18 %. The DPPH radical scavenging and Fe2+ chelating abilities were closely correlated with the peptide profiles and Ho of the hydrolysates. The peptide profiles of the hydrolysates with higher hydrophobic amino acids exhibited higher DPPH radical scavenging and Fe2+ chelating abilities.
关键词：hemp protein; hydrolysate; enzymatic hydrolysis; antioxidant activity; Neutrase®