文章基本信息

标题：Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003
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作者：Liu, Gao-Qiang ; Wang, Xiao-Ling
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2009
卷号：47
期号：2
页码：210-214
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：A practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the best carbon source, and that a combination of wheat bran extract and yeast extract was the best nitrogen source for both biomass and IPS production. The effects of the four factors were further investigated using four-factor, three-level orthogonal test design. The best combination for the production of biomass and IPS was (in g/L): corn flour extract 15, glucose 6, yeast extract 3, and wheat bran extract 6. The maximum biomass and IPS in a 30-litre stirred tank bioreactor reached 11.30 and 0.72 g/L in the optimized medium, respectively. The use of two low-cost raw materials like corn flour and wheat bran extract as the major medium constituents could reduce the costs of biomass and IPS production.
关键词：Agaricus blazei; intracellular polysaccharide; mycelial growth; medium selection; submerged fermentation