文章基本信息

标题：Optimization of Media for Enhanced Glucoamylase Production in Solid-State Fermentation by Fusarium solani
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作者：Bhatti, Haq Nawaz ; Rashid, Mohammad Hamid ; Nawaz, Rakhshanda 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2007
卷号：45
期号：1
页码：51-56
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Solid-state cultivation of Fusarium solani was carried out for enhanced production of glucoamylase (GA) using different substrates like wheat bran, rice bran, green gram bran, black gram bran and maize bran. The SSF medium containing wheat bran as a substrate yielded the highest enzyme activity. The physical and chemical parameters were optimized. Maximum enzyme activity (61.35±3.69) U/g of dry wheat bran was achieved under optimum growth conditions. The optimum conditions were fructose as carbon and energy additive 1 % (by mass), urea as nitrogen additive 1 % (by mass), initial moisture content of solid substrate 70 % (by mass per volume), incubation period 96 h, inoculum size 15 % (by mass per volume) having 10^6–10^7 spores/mL, incubation temperature (35±1) °C and pH=5.0. It was further observed that the addition of surfactants caused a decrease in enzyme biosynthesis by F. solani in SSF of wheat bran under optimum process conditions.
关键词：glucoamylase; wheat bran; optimization; solid-state fermentation; moisture content