文章基本信息

标题：Enhanced Production of Glucose Oxidase Using Penicillium notatum and Rice Polish
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作者：Sabir, Shazia ; Bhatti, Haq Nawaz ; Zia, Muhammad Anjum 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2007
卷号：45
期号：4
页码：443-446
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Glucose oxidase (GOD) is an important enzyme that finds a wide range of applications in food and pharmaceutical industry. In this investigation the feasibility of using rice polish as a substrate for the production of GOD by Penicillium notatum in submerged fermentation (SmF) has been evaluated. The intention was to enhance total GOD activity by the selection of economical substrate, microorganism and consecutive optimization of various cultural conditions. Maximum GOD activity of (112±5) U/mL was achieved under optimum growth conditions: rice polish 5 g, incubation period 72 h, buffering agent 3 % (by mass per volume), incubation temperature (30±1) °C and pH=6.0. Addition of carbon and nitrogen sources further enhanced the enzyme yield, indicating an economically attractive process for GOD production.
关键词：glucose oxidase; Penicillium notatum; submerged fermentation; rice polish; culture conditions