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标题：Production of Polyunsaturated Fatty Acids by Mucor recurvus sp. with Sugarcane Molasses as the Carbon Source
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作者：Li, Nan ; Deng, Zhi-Nian ; Qin, Yong-Ling 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2008
卷号：46
期号：1
页码：73-79
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production.
关键词：Mucor recurvus sp.; polyunsaturated fatty acids; sugarcane molasses; fermentation; cultivation condition; fatty acid analysis