文章基本信息

标题：Diversity of Y-Short Tandem Repeats in the Representative Sample of the Population of Canton Sarajevo Residents, Bosnia and Herzegovina
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作者：Ćenanović, Merisa ; Pojskić, Naris ; Kovačević, Lejla 等
期刊名称：Collegium Antropologicum
印刷版ISSN：0350-6134
出版年度：2010
卷号：34
期号：2
页码：545-550
出版社：Hrvatsko antropološko društvo
摘要：In our previous population study, we have used twelve Y-chromosomal short tandem repeats loci incorporated in the PowerPlex®Y System to determine Y-STR diversity in B&H human population. With intent to obtain additional verification of the previously obtained results as well as to establish specific reference for a local B&H population, we have decided to test DNA samples collected from 100 unrelated healthy male Canton Sarajevo residents (from Sarajevo region) for the same twelve Y-linked short tandem repeats loci. Qiagen DNeasyTM Tissue Kit (Qiagen, GmbH, Hilden, Germany) was used for DNA extraction from buccal swabs and PowerPlex®Y System (Promega Corp., Madison, WI) has been used to simultaneously amplify Y-STR loci by PCR. PowerPlex®Y System includes 12 STR loci: DYS19, DYS385a, DYS385b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. The total PCR reaction volume was 5 mL. PCR amplifications were carried out in PE GeneAmp PCR System Thermal Cycler (ABI). Electrophoresis of the amplification products was preformed on an ABI PRISM 310 genetic analyzer (ABI, Foster City, CA) according to the manufacturer’s recommendations. The raw data were compiled and analyzed using the accessory software: ABI PRISM®Data Collection Software and Genemapper® version 3.2. In addition, we have compared the obtained »Sarajevo« dataset with the data previously generated for the entire Bosnian and Herzegovinian population, as well as with the available data on geographically close (neighboring) European populations. The results of this study will be used as guidelines in additional improving of research into genetic relationship among recent local B&H populations, both isolated and open, which is a long term project in our country.
关键词：Key words: Y-STRs; population genetics; genetic diversity; PowerPlex®Y System; Canton Sarajevo; Bosnia and Herzegovina