文章基本信息

标题：Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
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作者：Petri Ihalainen ; Himadri Majumdar ; Tapani Viitala 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2013
卷号：3
期号：1
页码：1-17
DOI：10.3390/bios3010001
语种：English
出版社：MDPI Publishing
摘要：In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
关键词：paper electronics; nanoparticles; inkjet printing; immunoassays; impedance spectroscopy