The study is aimed at the investigation of trade implications for Pakistan in the milieu of globalisation. To this end, it examines the nature and application of WTO and identifies its operative tariff and non-tariff instruments. By doing so, it intends to trace out the place of Pakistan in the world market in comparison to its competitors. The study concludes that the pattern of trade preferences and WTO grants do not necessarily guarantee success in the export performance of the recipients. Similarly, various other demand and supply side factors also play an important role in this regard. Nevertheless, the main focus of the study remained on the investigation of trade implications of WTO for Pakistan’s Cotton exports and impact of EU enlargement on Cotton sector of Pakistan. The examination of comparative trade statistics denotes that Pakistan has been one of the leading trading partners of Cotton products for the world and proved to be a forefront supplier of selected Cotton items over the period under review. Although Pakistan’s export performance has been adequate in comparison to its competitors but still it needs to be accelerated