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标题：Supplementation of Indigenous Lactobacillus Bacteria in Live Prey and as Water Additive to Larviculture of Portunus pelagicus (Linnaeus, 1758)
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作者：A.D. Talpur ; A.J. Memon ; M.I. Khan 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2011
卷号：3
期号：5
页码：390-398
出版社：MAXWELL Science Publication
摘要：Experimental trials were conducted to demarcate the effects of indigenous Lactobacillus probiotics as bioencapsulated in live prey (rotifers Brachionus plicatilis and Artemia franciscana ) and water additives together on the survival of blue swimming crab, Portunus pelagicus larvae. Three LAB probiotics L. plantarum , L. salivarius and L. rhamnosus at final concentration 1x107 cfu/mL were bioencapsulated in live prey added daily and same allowance was added to culture water on day 1, 3, 5, 7, 9, 11, 13 as a single isolates to treatments A, B, C and multi isolates to D with no probiotic added to control tanks. Bacteria were successfully accumulated in both rotifers and Artemia within two hours of incubation. Total viable count of bacteria in Artemia observed lower at sampling days in inoculated tanks compared to those at time of incubation, contrary it increased in controls and no Vibrio was determined in Artemia in LAB mixture isolate inoculated samples on the day 13. Highest LAB bacteria 4.10×103 was determined in Artemia on day 11 in those inoculated with mixture of LAB isolates. At the end of the trials, larvae treated with a mixture of LAB probiotics did produce significantly highest survival 13.83±0.76% over other LAB treatments. As a single isolates L. plantarum did produce survival 13.50±1.32% compared with those treated with L. salivarius and L. rhamnosus and those without probiotics (control). There was no statistical significance (p>0.05) in the survival of larvae in any treatment. Results indicate that LAB probiotics could be used to enhance survival of P. pelagicus larvae.
关键词：Accumulated ; Artemia franciscana ; Brachionus plicatilis ; Lactobacillus ; significance ;