文章基本信息

标题：Remote control of DNA-acting enzymes by varying the Brownian dynamics of a distant DNA end
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作者：Hua Bai ; James E. Kath ; Felix Manuel Zörgiebel 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2012
卷号：109
期号：41
页码：16546-16551
DOI：10.1073/pnas.1203118109
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Enzyme rates are usually considered to be dependent on local properties of the molecules involved in reactions. However, for large molecules, distant constraints might affect reaction rates by affecting dynamics leading to transition states. In single-molecule experiments we have found that enzymes that relax DNA torsional stress display rates that depend strongly on how the distant ends of the molecule are constrained; experiments with different-sized particles tethered to the end of 10-kb DNAs reveal enzyme rates inversely correlated with particle drag coefficients. This effect can be understood in terms of the coupling between molecule extension and local molecular stresses: The rate of bead thermal motion controls the rate at which transition states are visited in the middle of a long DNA. Importantly, we have also observed this effect for reactions on unsupercoiled DNA; other enzymes show rates unaffected by bead size. Our results reveal a unique mechanism through which enzyme rates can be controlled by constraints on macromolecular or supramolecular substrates.
关键词：DNA topology ; DNA–protein interactions ; enzyme kinetics ; molecular friction ; single-molecule biophysics