文章基本信息

标题：Topography of funneled landscapes determines the thermodynamics and kinetics of protein folding
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作者：Jin Wang ; Ronaldo J. Oliveira ; Xiakun Chu 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2012
卷号：109
期号：39
页码：15763-15768
DOI：10.1073/pnas.1212842109
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The energy landscape approach has played a fundamental role in advancing our understanding of protein folding. Here, we quantify protein folding energy landscapes by exploring the underlying density of states. We identify three quantities essential for characterizing landscape topography: the stabilizing energy gap between the native and nonnative ensembles {delta}E, the energetic roughness {Delta}E, and the scale of landscape measured by the entropy S. We show that the dimensionless ratio between the gap, roughness, and entropy of the system [IMG]/medium/pnas.1212842109eq1.gif" ALT="Formula "> accurately predicts the thermodynamics, as well as the kinetics of folding. Large {Lambda} implies that the energy gap (or landscape slope towards the native state) is dominant, leading to more funneled landscapes. We investigate the role of topological and energetic roughness for proteins of different sizes and for proteins of the same size, but with different structural topologies. The landscape topography ratio {Lambda} is shown to be monotonically correlated with the thermodynamic stability against trapping, as characterized by the ratio of folding temperature versus trapping temperature. Furthermore, {Lambda} also monotonically correlates with the folding kinetic rates. These results provide the quantitative bridge between the landscape topography and experimental folding measurements.
关键词：energy landscape theory ; biomolecular dynamics