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标题：Diversity and distribution of fishes of Gaji River, Bauchi State, Nigeria
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作者：Ahmad J. Nayaya ; M. Nor Siti-Azizah ; Innocent A. Adikwu 等
期刊名称：Advances in Environmental Sciences - International Journal of the Bioflux Society
印刷版ISSN：2066-7620
电子版ISSN：2065-7647
出版年度：2012
卷号：4
期号：02
页码：50-58.
出版社：Bioflux
摘要：Fish sampling was conducted along the Gaji River with a view to assessing the Diversity and Distribution of the fishes. The river which is the major source of water for the diverse wild fauna in and around the Yankari National Park, is located on Latitude 9o 45’ 16” and Longitude 10o 30’ 37” and covers an area of about 2,244 km² (870 mi²) and is home to several natural warm water springs, as well as a wide variety of flora and fauna (Wikipedia 2011). A total of twenty sampling locations were established on the river and throughout the period of the work, different fishing gears which included cast nets, gill nets, entrance traps, hooks and line and in some cases seines were used. Furthermore, Mitochondrial DNA cytochrome b sequencing was employed to study the genetic diversity of Clarias gariepinus sampled from the study area due to its economic importance. Amplification of the cytochrome b (cyt b) gene was done using the polymerase chain reaction (PCR). A portion of mtDNA containing the gene was amplified using primers L15267, 5’-AAT GAC TTG AAG AAC CAC CGT-3’ and H15891, 5’-GTT TGA TCC CGT TTC GTG TA-3’ described by Briolay et al (1998). A total of 13 species were identified from the 4,218 individuals collected, representing 12 families and 13 genera. Their percentage distribution showed that Oreochromis niloticus had the highest percentage occurrence of about 31% followed by Parachanna obscura with 27%. This was followed by Clarias gariepinus which had about 20% distribution of occurrence. On the distribution of the identified species in the various sampling locations, Ruwan Bangiya had the highest occurrence of the different species with 11 out of the total 13 species found in this location. Ruwan Dinya, Ruwan Sarki and Ruwan Gajin Gwaza also had relatively high occurrence of the identified fish species. The lowest occurrence was found in Ruwan Dalamiri, Ruwan Kunkuru and Ruwan Kankwana. Ruwan Gada and Ruwan Kakkida had moderate distribution of the various species. Three haplotypes were identified and the result further showed that population distribution of C. gariepinus is homogenous throughout the study area during the study period. The mean genetic distance between all populations is low indicating that frequent gene flow occurs between all populations.
关键词：Nigeria; haplotype; PCR; fish diversity.