文章基本信息

标题：Extraction of Genomic DNA Using Magnetic Nanoparticles (Fe₃O₄) as a Solid-Phase Support
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作者：Z. M. Saiyed ; C. N. Ramchand
期刊名称：American Journal of Infectious Diseases
印刷版ISSN：1553-6203
出版年度：2007
卷号：3
期号：4
页码：225-229
DOI：10.3844/ajidsp.2007.225.229
语种：English
出版社：Science Publications
摘要：Magnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to the conventional procedures in all the samples tested. Additionally, the magnetic method takes less than 15 minutes to extract DNA as against several hours taken by conventional protocols. Furthermore, the isolated DNA was found to function satisfactorily in PCR amplification and restriction endonuclease digestion. The developed procedure is simple, quick, cheap, robust and does not require the use of organic solvents or sophisticated equipments; thereby making it more amenable to automation.
关键词：magnetic nanoparticles; mammalian cells; PCR; agarose gel