文章基本信息

标题：Nonlinear Dielectric Spectroscopy as an Indirect Probe of Metabolic Activity in Thylakoid Membrane
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作者：Jie Fang ; Akilan Palanisami ; Kimal Rajapakshe 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2011
卷号：1
期号：1
页码：13-22
出版社：MDPI Publishing
摘要：Abstract: Nonlinear dielectric spectroscopy (NDS) is a non-invasive probe of cellular metabolic activity with potential application in the development of whole-cell biosensors. However, the mechanism of NDS interaction with metabolic membrane proteins is poorly understood, partly due to the inherent complexity of single cell organisms. Here we use the light-activated electron transport chain of spinach thylakoid membrane as a model system to study how NDS interacts with metabolic activity. We find protein modification, as opposed to membrane pump activity, to be the dominant source of NDS signal change in this system. Potential mechanisms for such protein modifications include reactive oxygen species generation and light-activated phosphorylation.
关键词：nonlinear dielectric spectroscopy; reactive oxygen species; whole cell biosensor; thylakoid