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标题：Use of anti-idiotypic antibodies as cell-surface receptor probes
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作者：Karin Sege ; Per A. Peterson
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：5
页码：2443-2447
DOI：10.1073/pnas.75.5.2443
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Anti-idiotypic antibodies have been raised against antibodies to retinol-binding protein (RBP) and to insulin. After absorption the anti-idiotypic antibodies recognized the antigen-combining sites of the antibodies used as the immunogen but of no other antibodies. Some of the anti-idiotypic antibodies raised against antibodies to RBP bound specifically to rat intestine epithelial cells, which have a physiological cell-surface receptor for RBP. The RBP receptor mediates the uptake of retinol from RBP to the cells. This uptake was abolished in a concentration-dependent manner by the anti-idiotypic antibodies, which obviously competed with RBP for binding to the receptor. Anti-idiotypic antibodies against antibodies to insulin inhibited the binding of 125I-labeled insulin to isolated rat epididymal fat cells, whereas anti-idiotypic antibodies raised against antibodies to RBP had no effect. Furthermore, on interacting with young rat thymocytes, anti-idiotypic antibodies against antibodies to insulin stimulated the uptake by the cells of -aminoisobutyric acid, thereby mimicking the effect of insulin. These results suggest that in some cases anti-idiotypic antibodies may be useful tools in elucidating structure-function relationships for cell-membrane receptors.
关键词：retinol-binding protein ; insulin receptor ; insulin effect