文章基本信息

标题：Uracil incorporation into nascent DNA of thymine-requiring mutant of Bacillus subtilis 168
本地全文：下载
作者：Fuyuhiko Tamanoi ; Tuneko Okazak
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：5
页码：2195-2199
DOI：10.1073/pnas.75.5.2195
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A thymine-requiring mutant of Bacillus subtilis strain 168 accumulates short DNA chains after brief pulses with [3H]thymidine. Reversion of the thy mutation to thy+ abolishes the accumulation of short DNA chains, suggesting that the accumulation is related to the thy mutation. The reason for this accumulation has been further investigated by analysis of a mutant with a defective uracil-DNA glycosidase activity (urg). The accumulation of short DNA chains in thy- cells is abolished by the deficiency of uracil-DNA glycosidase activity. In thy+ cells, the deficiency of the glycosidase activity does not change the sedimentation profile of pulse-labeled DNA. DNA isolated from thy-urg- cells is fragmented by successive treatment with purified uracil-DNA glycosidase and alkali, indicating that uracil residues are present in this DNA. DNA isolated from thy+urg- cells is not fragmented by the same treatment. Significant radioactivity is detected in the dUMP region, when [3H]uridine-labeled DNA from thy-urg- cells is hydrolyzed and analyzed by thin-layer chromatography. Only a trace amount of radioactivity, which is not influenced by the deficiency of uracil-DNA glycosidase activity, is found in the dUMP region in DNA hydrolysates from thy+ cells. These results suggest that, in thy- cells, uracil is incorporated into DNA and the accumulation of short DNA chains results from the excision-repair of this uracil whereas in thy+ cells, uracil is seldom, if ever, incorporated into DNA.
关键词：accumulation of short DNA chains ; thy ^- and thy ^- cells ; uracil-DNA glycosidase ; deoxyuridylate