文章基本信息

标题：Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts
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作者：K Bose ; P Karran ; B Strauss 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：2
页码：794-798
DOI：10.1073/pnas.75.2.794
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Alkali-labile lesions introduced into T7 DNA by treatment with methyl methanesulfonate were removed and the DNA was repaired by incubation with DNA polymerase alpha and nuclease from a human lymphoblastoid line followed by the addition of DNA ligase. The nuclease preparation contains both apurinic endonuclease and 5'-3' exonuclease activities. Dinucleotides appear to be the first product of exonuclease action. Repair of methyl methanesulfonate-induced damage can occur by the insertion of only a few nucleotides per lesion as in vivo.