文章基本信息

标题：Regulatory function of simian virus 40 DNA replication for late viral gene expression
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作者：A Graessmann ; M Graessmann ; C Mueller 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：11
页码：4831-4834
DOI：10.1073/pnas.74.11.4831
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Inhibition of simian virus 40 (SV40) DNA synthesis prevents late but not early viral gene expression in infected cells. To test whether the late SV40 template specificity is elicited by replicative intermediate DNA molecules (RI-DNA), we isolated subcellular fractions containing RI-DNA from SV-40-infected monkey cells and microinjected these preparations into various cell lines under conditions in which viral DNA synthesis was blocked. Late SV40 gene expression (V-antigen synthesis) was obtained by microinjection of wild-type RI-DNA or temperature-sensitive mutant A7 RI-DNA preparations at 37 degrees or 41.5 degrees, respectively, while SV40 native superhelical DNA (DNA I) at a 10-fold higher concentration failed to induce V-antigen synthesis under the restrictive conditions. V-antigen synthesis was also obtained by microinjection of partially denatured SV40 DNA or a high number of randomly nicked DNA molecules (DNA II) in the absence of DNA synthesis; both single-stranded regions and nicks are specific features of RI-DNA molecules.