文章基本信息

标题：Structure of ribulose-1,5-bisphosphate carboxylase-oxygenase: Form III crystals
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作者：Timothy S. Baker ; Se Won Suh ; David Eisenberg 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：3
页码：1037-1041
DOI：10.1073/pnas.74.3.1037
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A new crystal form (III) of tobacco leaf ribulosebisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39 ] has been grown by dialysis procedures, and is suitable for structural studies at near atomic resolution. The crystals exhibit birefringence, grow as pseudo-regular rhombic dodecahedrons, and belong to the tetragonal space group P42212 with a = b = 149 A, c = 138 A, and V = 3.04 x 106 A3. Each unit cell contains two molecules, with two large and two small subunits per asymmetric unit. At low resolution (>10 A) the crystal structure is body centered belonging to space group 1422 with one large/small pair in the asymmetric unit. Thus, at low resolution the molecular symmetry is D4, the highest possible symmetry for an oligomer of stoichiometry large8small8. Form III crystals may be identical to crystalline inclusions found in chloroplasts.
关键词：protein structure ; x-ray diffraction ; molecular symmetry ; chloroplast crystals