文章基本信息

标题：Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease
本地全文：下载
作者：P Bhattacharya ; J R Moskal ; S Basu 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：3
页码：842-845
DOI：10.1073/pnas.74.3.842
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Activity of a trypsin-sensitive acid deoxyribonuclease (DNase) inhibitor has been detected in 13- to 21-day-old embryonic chicken brains and in clonal lines of neuroblastoma cells (adrenergic, N1E-115, and neurotransmitter-inactive, N-18) grown for 48 hr after subculture. The activities of purified porcine and bovine spleen DNases were inhibited 60--75% in the presence of the inhibitor, whereas less than 10% inhibition was observed with purified pancreatic DNase. Activities of an acidic DNase and its inhibitor reached maxima in 21-day-old embryonic chicken brain. The proteins were separated by isoelectric focusing and affinity column chromatographic techniques. The pI values of the acid DNase and its inhibitor were 5.4 and 4.2, respectively.